液相微萃取-高效液相色谱联用分析化妆品中痕量的雌激素

Determination of Trace Estrogens in Cosmetic Water by Liquid-Phase Microextraction Coupled with High Performance Liquid Chromatography

建立了液相微萃取-高效液相色谱联用(LPME-HPLC)测定爽肤水中痕量的雌三醇、雌二醇、炔雌醇和雌酮的分析方法,研究了萃取溶剂种类、接受相体积、搅拌速度、萃取时间等对萃取效率的影响。结果表明,该方法对4种雌激素的富集倍数可达到247-343倍,方法的线性范围为1-200μg/L,检出限为0.4-1.0μg/L,6次平行测定的相对标准偏差为3.6%-7.3%,爽肤水中的加标回收率为101.2%-114.9%。方法简单快速、灵敏度高、环境友好,满足痕量雌激素分析的要求。

Liquid-phase microextraction （LPME） was introduced to establish a two-phase system in solvent microextraction with the integrated features of sampling, extraction, concentration and injection in a single step. It was proved that this process is a fast, effective and lowcost sample preparative method and widely used in biomedical, environmental and food analysis, as well as in other fields. In this study, a rapid method for the determination of trace estrogens （ethinyl estradiol, estradiol, estriol, and estrone ） in cosmetic water by LPME coupled with high performance liquid chromatography （HPLC） was developed. The extraction condi- tions of LPME, such as extraction solvent, solvent volume, stirring speed, extraction time, pH value and salt concentration of the donor phase, were optimized. The enrichment factors of the estrogens was from 247 to 343. The linear detection ranges of the method were from 2 to 200μg/L with the detection limits from 0.4 to 1.0μg/L. The recoveries ranged from 101.2% to 114.9% and the relative standard deviations were less than 7.5% for cosmetic water samples. The method is suitable for the determination of trace estrogenic compounds in cosmetic water samples.