摘要
目的:研究黄连中小檗碱型生物碱的最佳提取工艺。方法:采用正交试验设计,以小檗碱、巴马汀、黄连碱、表小檗碱和药根碱的总含量为考察指标,考察乙醇浓度、提取时间、提取次数、乙醇用量4个因素对提取结果的影响。结果:最佳提取工艺为8倍量的50%乙醇,回流提取4次(每次2h)。结论:本工艺重现性好,测定方法可行,为大批量黄连提取生物碱提供了理论依据。
OBJECTIVE： To study the optimized process for extraction of the alkaloids from Rhizoma Coptidis. METHODS： Orthogonal design was used for studying the effect of alcohol concentration, volume, extraction time and times on the content of iatrorrhizine, epiberberine, coptisine, palmatine and berberine. RESULTS： The optimum extraction process is as follows： 8 times of 50% alcohol, extracted for 4 times, each time for 2 hours. CONCLUSION： This extraction process is workable and reproducible, and can provide theoretic support for mass extraction of the alkaloids from Rhizoma Coptidis.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第9期670-672,共3页
China Pharmacy
关键词
黄连
生物碱
正交试验
Rhizoma Coptidis
Alkaloids
Orthogonal test
