摘要
目的:研究黄连中小檗碱型生物碱的最佳提取工艺。方法:采用正交试验设计,以小檗碱、巴马汀、黄连碱、表小檗碱和药根碱的总含量为考察指标,考察乙醇浓度、提取时间、提取次数、乙醇用量4个因素对提取结果的影响。结果:最佳提取工艺为8倍量的50%乙醇,回流提取4次(每次2h)。结论:本工艺重现性好,测定方法可行,为大批量黄连提取生物碱提供了理论依据。
OBJECTIVE: To study the optimized process for extraction of the alkaloids from Rhizoma Coptidis. METHODS: Orthogonal design was used for studying the effect of alcohol concentration, volume, extraction time and times on the content of iatrorrhizine, epiberberine, coptisine, palmatine and berberine. RESULTS: The optimum extraction process is as follows: 8 times of 50% alcohol, extracted for 4 times, each time for 2 hours. CONCLUSION: This extraction process is workable and reproducible, and can provide theoretic support for mass extraction of the alkaloids from Rhizoma Coptidis.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第9期670-672,共3页
China Pharmacy
关键词
黄连
生物碱
正交试验
Rhizoma Coptidis
Alkaloids
Orthogonal test