摘要
目的:建立以高效液相色谱法测定咽炎颗粒中大黄素含量的方法。方法:色谱柱为Diamonsil C18(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水溶液(68∶32),检测波长为254nm。结果:大黄素进样量在0.26~3.90μg范围内线性关系良好(r=0.9999),平均回收率为96.73%(RSD=1.49%)。结论:本法准确、灵敏、重现性好,操作简便、可用于控制咽炎颗粒的质量。
OBJECTIVE: To establish an HPLC method for determination of Emodin in Yanyan granules. METHODS: The stationary phase was Diamonsil C18( 150mm×4.6mm,5μm) column and the mobile phase was methanol - 0.1% phosphoric acid(68 : 32), with detection wavelength set at 254nm. RESULTS: Within the range of 0.26-3.90/zg Emodin had a good linearity, and the average recovery was 96.73% (RSD = 1.49% ) .CONCLUSION: The method is accurate, sensitive, reproducible,simple and can be used for the quality control of Yanyan granules.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第9期684-686,共3页
China Pharmacy