低频振动对骨髓基质细胞成骨能力影响及机制的研究

Influence of low frequency vibration on osteogenesis of bone marrow stromal cells and its mechanism

目的了解低频振动对骨髓基质细胞(BMSCs)成骨能力及其OPG基因、RANKL基因表达的影响。方法分离、培养10周龄雌性SD大鼠BMSCs,随机分为静止培养对照组和振动组,振动组于培养的第11天开始接受振动干预7d;振动结束后对两组BMSCs的增殖、碱性磷酸酶(ALP)、钙化结节及OPGmRNA、RANKLmRNA进行检测。结果振动组BMSCs增殖能力较对照组明显提高(P<0.05),但ALP活性、钙化结节无明显改变。振动组BMSCsOPG基因表达明显上调(P<0.05),RANKL基因表达无明显变化。结论低频振动可促进BMSCs的增殖,可能与其促进OPG基因表达上调有关;但未发现能使BMSCs成骨能力出现明显改变。

Objective To explore the effect of low frequency vibration （LFV） on osteogenesis of bone marrow stromal cells （BMSCs） and OPG mRNA,RANKL mRNA expression. Methods BMSCs from 10-week-old female SD rats were cultured and randomly divided into 2 groups： control group and vibration group. The BMSCs of vibration group was given LVF with 20 Hz, 0.25 g, 2 times/d, 10 min/time for 7 days. The LFV was given from the eleventh day after BMSCs cultured. The osteogenesis and OPG mRNA, RANKL mRNA of all BMSCs in two group were tested at the end of LFV. Results Compared to the control groups, the BMSCs growth and proliferation improved in vibration group, but the alkaline phosphatase （ALP） activity and the number of mineral nodule did not changed significantly. The OPG mRNA elevated in vibration group, while the RANKL mRNA showed no change. Conclusion The LFV may increase the growth and proliferation of BMSCs, which is attributed to the elevated OPG gene expression. The osteogenesis improved no significance, which may be associated with the too short vibration time.