蛇床子素对南瓜白粉病菌侵染的影响

Effect of Osthol on the Invasion of Sphaerotheca fuliginea

采用室内离体叶片法,发现蛇床子素(osthol)处理能显著降低南瓜白粉病菌侵染的病情指数,接菌6d后对照的病情指数为68.89,经100μg/mL蛇床子素处理组的病情指数仅为15.56。显微观察发现蛇床子素可有效降低南瓜白粉病菌对寄主的入侵,并可有效抑制菌丝的生长。接菌后48h,对照菌丝的平均长度接近400μm,蛇床子素处理组菌丝平均长度仅有122μm。苯胺蓝染色后显微观察发现蛇床子素还可降低白粉病菌的产孢量。

The effect of osthol on the development of Sphaerotheca fuliginea was studied by detached leaves. The results showed that 6 days after inoculation, the disease index was 68.89 in the control, while that was only 15.56 in the treatment with osthol at 100 μg/mL. The number of interaction sites with H2O2 accumulation was strongly decreased in the leaves treated with osthol. Osthol also depressed the growth of mycelia. The average colony length was about 400 μm in the control, while that was only 122 μm in the treatment with 100 μg/mL osthol. Osthol could also depress conidiation of powdery mildew.