电子探针在分析农作物叶片元素含量及微区分析中的应用

Application of Electronic Probe on Element Content Measurement in Plant Leaves and Microzone Analysis

对农作物进行元素分析是农业生产中最为重要的内容之一。目前,用于元素分析的方法很多,但多数方法都存在样品处理复杂以及很难对叶片表面微区进行元素分析等缺点。本文通过电子探针对土壤和4种植物的叶片细胞内的元素组成和含量进行了分析,结果表明,电子探针可以很好地将叶片内元素种类和含量测定出来。植物叶片元素含量与土壤中元素含量不一定相关。线扫描和面扫描分析看出,电子探针可以快速对叶片表面特定区域以及特殊结构进行元素种类和含量分析。

There are many methods for analyzing the element content, which are complicated in sample-treatment and cannot be used for analyzing the element content in the leaf surface microzones. The electronic probe method has the merit of simple pre-treatment without sample destruction and could be used to analyze the element content and their distribution in microzones, therefore it is widely used in metallurgical, geological, archeological fields. In this paper, we analyzed the element composition and contents in soil and mesophyll cells of four plant species by using electronic probe. The results showed that the element composition and contents in mesophyll cells could be well measured through this method. The element contents in mesophyll cells were not certainly correlated with those in the soil, which indicated that the elements absorption of plants was determined by its heredity and also affected by the element content of the soil. The linear scanning method can be show the element content in every scanning point along the restricted line, indicating that the element content was varied remarkably in the different scanning points of leaves. The map scanning technique also could demonstrate the element content and their distribution in a particular region, for example, in the particle structure of leaf surface of B. napus. In conclusion, the electronic probe technology can be effectively used in analyzing element content in microzone of plant leaf surface.