小儿类风湿关节炎患者外周血CD4^+、CD8^+ T细胞CD_(69)表达及意义

Expression and significance of CD_(69) on peripheral CD4^+, CD8^+ T-cell in infants with rheumatoid arthritis

目的分析小儿类风湿关节炎(RA)患者的外周血CD4+、CD8+T细胞CD69表达水平,探讨其在小儿关节炎症发生过程中的免疫病理作用。方法收集32例RA患儿和30例健康儿童外周血标本,利用流式细胞仪检测CD4+、CD8+T细胞CD69表达,分析它们在疾病发展过程中的作用。结果RA患儿外周血CD4+、CD8+T细胞CD69表达明显升高,与健康者比较有统计学差异(P<0·05),所有患儿均接受了有效的药物治疗,待症状控制后,分析发现患者的CD4+、CD8+T细胞表达CD69水平显著下降(P<0·05)。结论RA患儿外周血T细胞表达CD69水平明显升高,提示T细胞在关节炎症病变过程中激活、增殖,参与关节滑膜组织及全身免疫应答。

Objective To determine the expression level of CD69 on CD4^＋ and CD8^＋ T cells in peripheral blood from children with rheumatoid arthritis （RA）, and investigate their role in the pathogenesis of RA, Methods Peripheral blood was taken from 32 infants with RA and 30 healthy controls, Expression of CD69 on CD^4 ＋ and CD8^ ＋ T cells was detected on a flow eytometry. Results Expression of CD25 and CD69 was significantly increased on CD4^＋ and CD8^＋ T cells in peripheral blood of RA children, as compared with that in healthy controls （ P 〈 0,05 ）, Importantly, the expression of CD69 expression on peripheral blood CD4^＋ and CD8^＋ T cells was markedly decreased after receiving effective treatment （ P 〈 0,05）. Conclusion The expression level of CD69 is significantly increased in peripheral blood T cells from RA children, suggesting that activated T cells may play a pathogenic role in the induction of immune response.