消瘿强肌汤治疗甲状腺功能亢进性肌病大鼠的形态学观察

MORPHOLOGICAL OBSERVATION OF THE EFFECT OF XIAOYINGQIANGJITANG ON THYROTOXIC MYOPATHY IN RATS

目的建立甲状腺功能亢进性肌病(甲亢肌病)的动物模型,观察消瘿强肌汤治疗后大鼠骨骼肌细胞的形态学变化。方法36只健康雌性Wistar大鼠随机分为4组:甲亢肌病模型组10只,腹腔注射甲状腺激素钠盐每天1次,共42 d;中药治疗组10只,腹腔注射甲状腺激素钠盐42 d后,灌服消瘿强肌汤30 d;生理盐水对照组10只,腹腔注射甲状腺激素钠盐42 d后,灌服生理盐水30 d。另外6只每天腹腔注射生理盐水作为正常对照组。取胫骨前肌、腓肠肌、趾长伸肌标本制成石蜡切片,行苏木精-伊红染色及苏木精-偶氮焰红染色,观察各组骨骼肌细胞形态学变化。结果苏木精-伊红染色显示,甲亢肌病模型组骨骼肌肌细胞肌膜下核明显增多,细胞间隙略宽,横纹模糊不清,有时可见肌纤维大小不等,肥大和萎缩的肌束交替排列或镶嵌状存在;而中药治疗组骨骼肌肌束排列较整齐,肌细胞核无明显增多。苏木精-偶氮焰红染色法观察显示,甲亢肌病模型组可见明显的肌束间结缔组织增多,染成绿色;中药治疗组结缔组织明显减少。结论灌服消瘿强肌汤可明显恢复甲亢肌病大鼠骨骼肌的结构。

Objective To establish an animal model of thyrotoxic myopathy, and to observe the morphological changes of its skeletal muscle cells after treatment with Xiaoyingqiangjitang （XYQJT,a traditional Chinese medicine）. Methods Thirty-six Wistar rats were randomly divided into four groups： group Ⅰ received intraperitoneal injection of L-thyroxine for 42 days to establish a model of thyrotoxic myopathy; group Ⅱ was treated with XYQJT for 30 days after L-thyroxine intraperitoneal injection; group Ⅲ was given normal saline for 30 days after L-thyroxine intraperitoneal injection as control; group IV served as normal control group. The skeletal muscles from the legs were removed and made into paraffin sections and stained by hematoxylin-eosin （HE） and hematoxylin- Azocarmine （HA）. The morphology was observed made microscopically, and levels of serum T3 and T4 were also detected. Results After injection of L-thyroxine for 42 days, more nuclei under sarcolemma were observed; the spaces between the muscle fibers became slightly widen, the striations became blurred. Alter receiving XYQJT, all the abnormalities stated above disappeared. HA staining showed that the connective tissue between the muscle fibers in group Ⅰ was apparently abundant, while much less in group Ⅱ. Conclusion XYQJT can obviously recover the structure of skeletal muscles caused by thyrotoxic myopathy in rats.