人肝癌组织中nm23-H1基因突变的检测及意义

NM23-H1 GENE MUTATION IN HUMAN HEPATOCELLULAR CARCINOMA: ITS DETECTION AND SIGNIFICANCE

目的观察肿瘤转移抑制基因nm23-H1在人原发性肝细胞癌中的突变情况,探讨nm23-H1基因突变与肝癌发生、发展及转移的关系。方法采用聚合酶链反应-单链构象多态性技术(PCR-SSCP),对16例肝癌组织、12例癌旁组织和4例正常肝组织的nm23-H1基因的第1、2、4外显子突变情况突变进行检测。结果肝癌组织中有1例nm23-H1基因第1外显子纯合缺失;肝癌组织中1例第1外显子、1例第2外显子,癌旁组织中2例第2外显子有单链DNA泳动变位。结论nm23-H1基因突变在肝癌组织中发生率低。

Objective To investigate the mutation of nm23-H1 gene and to explore the relationship between nm23-H1 mutation and the development, progression and metastasis of human hepatocellular carcinoma （HCC）. Methods Mutations of the exon1,2,4 of nm23-H1 gene in 16 HCC tissues, 12 adjacent liver tissues and four normal liver tissues were examined by polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP）. Results One of exon 1 homozygous loss of allele of nm23-H1 gene was observed in HCC tissue. The mobility shift of single strand DNA of nm23-H1 was also examined including one of exonl in HCC tissues, one of exon 2 in adjacent liver tissue and two of exon 2 in adjacent liver tissue. Conclusion The incidence of mutation of nm23-H1 gene in HCC is low.