食管鳞状细胞癌组织MMP-2、9与TIMP-1表达及意义

EXPRESSIONS OF MMP-2,MMP-9 AND THEIR TISSUE INHIBITOR TIMP-1 IN ESOPHAGEAL CARCINOMA AND THEIR CLINICAL SIGNIFICANCE

目的 探讨食管鳞状细胞癌组织中基质金属蛋白酶2、9（MMP-2、MMP-9）及其组织抑制因子（TIMP-1）的表达，并分析其与临床病理因素的关系。方法 应用免疫组织化学方法，检测55例食管鳞状细胞癌组织中MMP-2、MMP-9、TIMP-1的表达。同时，取10例癌周正常食管黏膜作为对照。结果 MMP-2、MMP-9、TIMP-1在正常黏膜上皮不表达；在食管鳞状细胞癌中阳性表达率分别为21．8％、81.8％和78．2％；不同组织学分级的食管癌组织中MMP-2、MMP-9及TIMP-1蛋白阳性表达率无显著性差异（Х^2=0.68～2．76，P〉0．05）；随着肿瘤浸润程度的加深，MMP-9阳性表达率明显升高，差异有显著性（Х^2=16．55，P〈0．05）；有淋巴结转移者MMP-9阳性表达率明显高于无淋巴结转移者，差异有显著性（Х^2=10．96，P〈0．05），且临床病理分期为Ⅲ期者明显高于0～Ⅰ及Ⅱ期（Х^2=4．89、9．46，P〈0．05）。结论 MMP-2、MMP-9、TIMP-1的表达与肿瘤分化程度无关．MMP-9表达与肿瘤浸润深度、淋巴结转移及临床分期有关。

Objective To investigate the expressions of matrix metallproteinase-2 （MMP-2） and -9（MMP-9） and tissue inhibitor of matrix metallproteinase-1 （TIMP-1） in squamous cell carcinoma of esophagus and their correlation with clinicopathological parameters. Methods Expressions of MMP-2, MMP-9 and TIMP-1 were detected by immunohistochemical staining in seetions of the carcinoma （n=55）. Results Expression of MMP-2, MMP-9 and TIMP-1 were observed in the tissue of the carcinoma, but not in normal esophageal tissue, with the positive rates of 21. 8%, 81. 8% and 78. 2%, respectively. Expressions of MMP-2, MMP-9 and TIMP-1 were not correlated with the histological differentiation （Х^2=0. 68-2.76, P〉0. 05）. Expression of MMP-9 was correlated with the degree of cancer invasion （Х^2= 16.55, P〈0.05）. The positive rate of MMP-9 expression was significantly higher in patients with lymph node metastasis than those wihout （Х^2= 10.96, P〈0.05）, also obviously higher in cases with clinicopathological stage Ⅲ than in those with 0-Ⅰ and Ⅱ （ Х^2=4.89,9.46, P〈0.05）. Conclusion Expressions of MMP- 2, MMP-9 and TIMP-1 are not correlated with histological differentiation, while expression of MMP-9 is related with tumor invasion and lymph node metastasis.