PTEN蛋白在胃癌、癌旁组织及癌前病变中的表达及意义

Expression of PTEN protein in gastric carcinoma, adjacent mucosa and precancerous lesions of stomach and its significance

目的比较胃癌、癌旁组织、肠上皮化生和异型增生组织中的PTEN蛋白表达阳性率,探讨PTEN在胃癌发生、发展中的作用。方法利用免疫组化法测定141例胃癌及癌旁组织、慢性胃炎肠化组织30例、慢性胃炎不典型增生组织55例的PTEN蛋白表达阳性率,以浅表性胃炎20例作为对照,比较胃癌的不同临床病理分期、淋巴结转移情况、组织学分型、癌旁组织与肠上皮化生和异型增生组织的PTEN蛋白表达阳性率有无差异。结果胃癌组织的PTEN蛋白表达阳性率低于肠上皮化生、异型增生组织及正常胃黏膜组织(P<0.05),但胃癌组织PTEN蛋白表达阳性率与肿瘤TNM分期无关(P>0.05)。黏液腺癌PTEN表达阳性率低于管状腺癌(P<0.05);有淋巴结转移者PTEN表达阳性率低于无淋巴结转移者(P<0.05);癌旁不典型增生组织的PTEN蛋白表达阳性率低于浅表性胃炎组织及肠化组织(P<0.05),与慢性胃炎中不典型增生组织相比差异无统计学意义(P>0.05);肠化组织的PTEN蛋白表达率与浅表性胃炎组织差异无统计学意义(P>0.05)。结论PTEN蛋白表达下调在胃癌的发生、发展中有重要作用,并与肿瘤细胞的生物学行为有一定关系。

Objective To compare the expression of phosphatase and tensin homologue derived from chromosome ten （PTEN）encoding product in carcinoma,its adjacent mucosa,intestinal metaplasia （IM）and dysplasia of the stomach, and to evaluate its clinical implication in tumorigenesis and progression of gastric carcinoma. Methods Tissue specimens from 141 cases of gastric carcinoma and its adjacent mucosa,30 cases of IM,55 cases of dysplasia and 20 cases of superficial gastritis were evaluated for the expression of PTEN by immunohistochemistry. Results The positive rate of PTEN protein in gastric carcinoma was lower than that of IM,dysplasia and superficial gastritis （P〈 0.05 ）,but it was not related with tumor stage （P〉0.05）. The positive rate of PTEN protein in mucoid adenocarcinoma was lower than that of ductal adenocarcinoma （P〈0.05）. PTEN was less expressed in gastric carcinoma with lymph node metastasis than that without （P〈0.05）. The positive rate of PTEN protein in dysplasia of gastric carcinoma adjacent mucosa was lower than those of superficial gastritis and IM （P〈0.05）, but without any difference compared to dysplasia in chronic gastritis（P〉0.05 ）. There was no difference in positive rate of PTEN protein between IM and superficial gastritis （P〉0.05）. Conclusion Reduced expression of PTEN protein might be important in tumorigenesis and progression of gastric carcinoma and it is associated with the biological behavior of the tumor ceils.