血管内皮生长因子促进移植脂肪血管增生的实验研究

The promotion of angiogenesis in transplanted fat tissue with vascular endothelial growth factor

目的了解血管内皮生长因子(VEGF)对移植脂肪的促血管增生作用。方法切取兔腹股沟脂肪并剪成颗粒状。在兔背部两侧各作一腔隙,将1μgVEGF注射入一侧腔隙筋膜下,在腔隙中再注入脂肪颗粒,对侧仅注入脂肪颗粒作为对照。15d后收集移植脂肪,作肉眼和镜下观察,计算血管数。结果实验侧血管增生明显多于对照侧,差异有统计学意义(P<0.01)。结论VEGF可以促进移植脂肪组织的血管增生,可能有提高其生存率的作用。

Objective To investigate the angiogenetic effect of vascular endothelial growth factor （VEGF） on transplanted fat tissue. Methods Fat tissue was harvested from the groin of rabbits and cut into grain. Two subcutaneous cavities were made in each side of dorsal medial line of the rabbits. VEGF was injected to the sub-fascia of one cavity and then fat tissue was transplanted into both cavities. The transplanted fat was taken out at 15d and observed with macroscopically and microscopically. The microvessels were counted by medical imagine analysis system. Results The density of microvessels in the experiment group was much higher that that in the control group （P〈0.01）. Conclusion VEGF can promote angiogenesis in transplanted fat tissue.