摘要
以SephadexLH-20柱色谱分离得到茶黄素双没食子酸酯(TFDG)成分,以H1299和HCT-116细胞分析了该成分的抗癌活性及其作用机理。结果表明,TFDG具有良好的抑制H1299细胞生长的活性,IC50为25μmol/L;有调节细胞周期的活性,可增加HCT-116细胞G1期细胞的比例;有促进HCT-116细胞凋亡的作用,浓度为50μmol/L时效果显著,48h凋亡率达到40%以上；Western杂交技术分析结果表明,它可降低HCT-116细胞中促癌蛋白质因子Bcl-xL的表达量,可增加抑癌蛋白质因子Bax的表达量。
Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H 1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H 1299, and its IC50 value is 25 μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.
出处
《茶叶科学》
CAS
CSCD
北大核心
2007年第1期33-38,44,共7页
Journal of Tea Science
基金
国家自然科学基金(30570191)
浙江省自然科学基金(Y304477)研究内容
关键词
茶黄素
抗癌
细胞周期
凋亡
蛋白质印迹
theaflavin, anticancer, cell cycle, apoptosis, western blotting
