Survivin在大肠癌中的表达及其与Bcl-2、P53的关系

Expression of Survivin and Its Correlation with Expression of Bcl-2 and P53 in Colorectal Adenocarcinoma

目的:研究凋亡抑制因子Survivin蛋白在大肠癌中的表达及其与患者临床病理和预后的关系。检测大肠癌组织中Survivin的表达与Bcl-2,P53的表达的关系。方法:用免疫组织化学方法检测115例大肠癌组织中Survivin,Bcl-2及P53的蛋白表达,并对病例随访5年。结果:Survivin在大肠癌中的阳性表达率为74/115(64．3%)。正常肠黏膜组织不表达Survivin。Survivin与患者的临床病理特征无显著相关性(P＞0．05)。Survivin的表达率在Bcl-2阳性的患者明显高于Bcl-2阴性的患者(P＜0．001),但是和P53的表达无显著相关性(P＞0．05)。Survivin阳性的患者5年生存率明显低于Survivin阴性的患者(P=0．001)。结论:在大肠癌组织中检测Survivin对于肿瘤患者的预后以及基于抗凋亡机制的肿瘤靶向治疗都有很重要的意义。

Objective： To study the expression of apoptosis inhibitor protein of Survivin in colorectal adenocarcinoma （GAG） and its correlation with clinicalpathologic parameters and prognosis and to examine the relationship between the Survivin expression and the expression of Bcl-2 and P53 in colorectal adenocarcinoma. Methods： 115 cases of colorectal adenocarcinoma, by immnohistoclaemical staining, were examined survivin, Bcl-2, and P53. The cases were followed up for 5 years. Results： Survivin was detected in 74 of 115 cases （64.3%）. No expression of survivin was observed in normal tissues, There was no correlation between Survivin immnoactivity and clinicalpathologic parameters （P〉0.05）. Survivin expression was significandy higher in Bcl-2 positive patients than in Bcl-2 negadve cases （88.9% versus 42.6%, p〈0.001）, but was not associated with P53 （P〉0.05）. The 5-year survival rate for the patients with survivin positive was siginificandy lower than that for the patients with survivin negative （p=0.001）. Conclusions： Examining surviving in GAG tissue is of significance for tumor patients＇ prognosis and targeted therapy based on antiapoptosis mechanism.