鹿茸干细胞体外培养技术的研究

Cultural Technique of Deer Antler Stem Cell in Vitro

利用消化酶消化法,用DMEM培养基对鹿茸干细胞进行了体外培养,分别从原代培养、传代、冻存、以及复苏等几个环节进行了试验。摸索鹿茸干细胞合适的体外培养条件,为鹿茸的各项相关研究打基础。结果表明,用透明质酸酶和胶原酶对组织样进行消化,然后用10%DMEM培养基进行培养细胞生长效果较好。适宜培养条件是37℃、5%CO2最大饱和湿度。

The culture conditions of deer antler cell in vitro were studied in the research. The result showed that the cell could be cultured in vitro with the hyaluronidase and collagenase to digest tissue and grow well in the medium of 10% FCS DMEM. The optimized culture condition was in the environment of 37℃, 5 % CO2 and saturated humidity.