人类β干扰素对乙脑病毒在人类细胞系上增殖的抑制作用

Inhibition of Japanese Encephalitis Virus Growth in Human Cell Lines by Human Interferon-beta

本文研究了人类β干扰素对乙型脑炎病毒在３株人类细胞株上增殖的抑制作用（Ｈｅｐ－２，ＦＬ和Ｕ９３７）．人类β干扰素在１０００Ｉμ／ｍｌ时，病变产生在ＦＬ细胞，Ｈｅｐ－２细胞和Ｕ９３７细胞上分别是对照组的０．５％，２．７％，３７．２％。本研究同时比较了人类β干扰素在人细胞株，猴细胞株和地鼠细胞株上对乙脑病毒的抑制作用（Ｈｅｐ－２，Ｖｅｒｏ，ＢＨＫ２１）其敏感性有明确的差异（许多文献已有报道）。本研究提示了在人体内的不同细胞上对干扰素抑制病毒的作用亦会有差异。

The inhibition of the Japanese encephalitis (JE) virus growth by human interferon (IFN)-beta was studied in three human cell lines,ie,Hep-2,FL and U937 cell lines. The growth of JE virus Nabayama strain was more sensitive to human IFN-beta tn FL and Hep-2 cells than in U937 cell line,at 1,000 IU/ml concentration;the yield of JE virus was reduced to 0. 5% of that in the control in FL,2.7% in Hep-2,and 37. 2% in U937 cell line respectively. The IFN concentration to reduce the virus yield to 50% of the control in Hep-2 cell was about 62 IU/ml for JE virus in contrast to 31 IU/ml for Sindbis virus. The inhibitory effect of INF-beta on the growth of JE virus was mare pronounced in human cell line,Hep-2,than in African green monkey kidney cell line,Vero,while significant sensitivity was not observed in hamster kindney cell line,BHK21.