摘要
用RT-PCR(逆转录聚合酶链反应)扩增丙型肝炎病毒5′端非编码(5′NCR)序列,246例肝炎患者中HCVRNA(丙型肝炎病毒核糖核酸)阳性者45例,阳性率18.3%(45/246),凡阳性者用酶联免疫法测抗-HCV(抗丙型肝炎病毒抗体),其中24例测到抗-HCV,阳性重叠率53.3%(24/45),用RT-PCR法检测HCVRNA,能在抗-HCV转阳以前检出HCVRNA,更早地确诊丙型肝炎。
RT-PCR method was applied in detecting HCV-RNA by way of amplifying HCV 5'NCR sequence. 246 cases with hepatitis were, examined in this study and among them 45 cases were HCV-RNA positive,about 18,3% of the total.These 45 positive cases were also tested with ELISA,a presently common way for the diagnosis of Hepatitis C, to detect the antibody to HCV and the positive rate this time was only 24/45,The result of the study suggested that the RT-PCR method was more sensitive than the ELISA method to HCV detection and could be employed for better clinical diagnosis.
出处
《海南医学院学报》
CAS
1996年第4期162-164,共3页
Journal of Hainan Medical University