Proso millet醇溶蛋白调节小鼠血清胆固醇代谢的作用及其机理

Effect of Proso Millet Prolamin on Plasma Cholesterol Metabolism in Mice

通过比较分别饲喂不同蛋白源(酪蛋白、大豆分离蛋白、Proso m illet醇溶蛋白)饲料的小鼠血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、甘油三酯(TG)浓度,旨在评价Proso m illet醇溶蛋白对小鼠胆固醇代谢的特定作用,并通过体外实验探讨其作用机理。结果显示,以Prosom illet醇溶蛋白(添加1.83%Lys和0.23%Trp)为唯一氮源的小鼠日粮对TC、TG无明显作用,却能显著提高小鼠血清HDL-c浓度(P<0.05),降低小鼠动脉硬化指数(AI)。但在体外其并不能抑制羟甲基戊二酸单酰辅酶A(HMG-CoA)还原酶的活性,即不能直接抑制胆固醇的合成,所以,其对胆固醇代谢的调节作用另有其他途径。

The objective of this study was to evaluate the effect of proso millet prolamin concentrate （PMP） on cholesterol metabolism in mice, by comparing the concentration of serum total cholesterol （ TC）, high - density lipo- protein cholesterol （ HDL - c） , low - density lipoprotein cholesterol （ LDL - c） and triglyceride （TG） of three mice groups given diets respectively containing PMP （supplemented with 1.83% Lys and 0.23% Trp）, casein or soy protein isolation （SPI） as protein sources. As the results present, the mice group fed with PMP diet supplemented with Lys and Trp exhibits significantly higher HDL - c concentration （ P 〈 0.05 ） and the lowest atherogenic index （AI） among the three mice groups, without change of levels of TC and TG. In vitro, the PMP can not suppress the activity of 3 - hydroxy -3 - methylglutaryl - coenayme A （ HMG - CoA） reductase, which means PMP can not inhibit directly the synthesis of cholesterol. Maybe, PMP adjusts the metabolism of serum cholesterol by some other ways.