Ⅰ期细支气管肺泡癌和肺腺癌增殖凋亡和转移特性的研究

A study on the differences in proliferative, apoptotic and metastatic activities between the stage Ⅰ bronchioloalveolar carcinoma and adenocarcinoma ofthe lung

目的探讨新定义后的细支气管肺泡癌与肺腺癌生长和转移性的差异。方法采用流式细胞术检测15例工期细支气管肺泡癌和19例普通肺腺癌的DNA指数、增殖指数（PI）、总s期细胞比率（SPF）和凋亡细胞比率（PA），免疫组织化学检测E—Cadherin、Collagen Ⅳ和VEGFR2的表达。结果细支气管肺泡癌的PA大于普通肺腺癌，PI和SPF值在普通肺腺癌与细支气管肺泡癌中差异无统计学意义；细支气管肺泡癌的E-Cadherin、Collagen Ⅳ指标表达水平高于普通肺腺癌，VEGFR2在普通肺腺癌、细支气管肺泡癌中的表达差异无统计学意义。结论早期细支气管肺癌与普通肺腺癌相比，具有相同增殖水平，较高的凋亡水平和更低的转移倾向。

Objective To analyze differences in proliferative, apoptotic and metastatic activities between stage Ⅰ peripheral BAG and adenocarcinoma of the lung. Method Flow cytometry and immunohistochemistry examinations of paraffin-embeded specimens were perfomed in 15 patients with BAC and 19 patients with adenocarcinoma of the lung. Determination of the S-phase fraction, proliferative index, DNA index and proportion of apoptotic ceils to assess cancer ceil proliferation and apoptosis, and the expression of E-Cadherin, Collagen Ⅳ and VEGFR2 were used to assess cancer cell metastatic activity. Results The results of flow cytometry revealed no difference in distribution among the S-phase fraction, Proliferative index, DNA index, but Proportion of apoptotic cells in BAC was higher than that in ad Any differences in immtmohistochemical staining for VEGFR2 in adenocarcinoma and BAC. In BAC, the level of expression for E-Cadherin, Collagen IV was higher than that in adenocarcinoma. Conclusion BAC had higher apoptotic activity and lower metastatic activity than common adenocarcinoma of the lung.