甜菜碱醛脱氢酶基因转化速生杨107的研究

Transformation of Populus×euramericana 'Neva' with Betaine-aldehyde Dehydrogenase Gene

利用农杆菌介导法将甜菜碱醛脱氢酶(BADH)基因导入速生杨107号中,以提高其耐盐性。对650个叶盘进行抗生素筛选获得25株抗性芽,PCR检测表明,BADH基因已整合到其中10株的基因组中。耐盐筛选结果表明,转基因植株在NaCl浓度为50、100 mmol/L的生根培养基上生长情况均好于未转化植株;相对电导率测定结果表明,在同等盐胁迫下转基因植株细胞膜较未转化植株能更好的保持其完整性。

Betaine-aldehyde dehydrogenase （BADH） gene was transferred into Populus × euramer/cana ‘Neva＇ by Agrobacter/ummediated transfomation in order to enhance the salt tolerance. Twenty-five buds survived after antibiotic screening of 650 leaf discs, the following PCR analysis proved that the BADH gene had been integrated into plant genome in 10 of the 25 buds. The salt tolerance test suggesled that the transgenic buds grew better in root inducing medium eontaining 50 and 100 mmol/L NaCl in compere with nontransformed buds. In addition,the transgenic plants showed better integrity of cell membrane than wildtype in the same condition of salt stress, which were demostrated by relative conductivity determination.