不同浓度大豆分离蛋白热诱导聚集体的研究

Research on heat-induced soy protein isolate aggregates

采用体积排阻色谱(SEC-HPLC)和激光光散射(LLS)研究了由醇洗豆粕制备的不同浓度的大豆分离蛋白热诱导聚集体(100℃,15 min)的分子量分布和粒径分布。SEC-HPLC检测结果表明,经热处理的蛋白溶液主要由3部分组成,即聚集体、中间体及未聚集部分;蛋白浓度为1%时,聚集体的百分含量为18.70%;蛋白浓度增加到5%时,聚集体的百分含量增加到54.15%;同时LLS的测定结果表明,蛋白溶液有不均一的粒径分布且体系浓度增加时平均粒径(Rh)由56.5 nm增至144.9 nm。

Molecular weight distribution and particle size distribution of heat - induced soy protein isolate aggregates prepared from alcohol washed soy meal were researched by high performance size exclusion chromatography （ SEC - HPLC ） and laser light scattering （LLS）, respectively SEC - HPLC analysis showed that the heated protein solutions were composed of three major parts, i. e. , aggregate, intermediate and unaggregated molecules, and with the protein concentration increased from 1% to 5%, the aggregates fraction increased from 18.70% to 54.15%. At the same time,LLS analysis showed that there were inhomogeneous particle size distribution in protein solution, and with the protein concentration increased, the average hydrodynamic radius （Rh） increased from 56.5 nm to 144.9 nm.