硅溶胶-膨润土法澄清大蒜多糖提取液的研究

Optimum Parameters for Clarifying Extract of Garlic Polysaccharide by Using Acidic Silica Sol and Bentonite

为找出一种既经济又快速的澄清大蒜多糖提取液的方法,以透光率、沉淀率、考马斯亮兰反应为指标,评价不同的pH值、不同酸性硅溶胶和膨润土用量对大蒜多糖提取液的澄清效果,然后选用效果较好的因素水平做正交实验。结果表明:在pH4的环境下,加入0.1%酸性硅溶胶和0.2%膨润土,可除去蛋白质等杂质。

Garlic polysaccharide is a new kind of fructan. In order to produce this commercial fructan, the economical and fast method to clarify the fructan extract was investigated. The acidic silica sol was prepared from the sodium silicate （commercial water glass） by the ion cation exchange resin. The acidic silica sol and the bentonite at the different concentrations were added into the garlic polysaccharide extract at the different pH values. The results shown that when at pH 4, the adding quantity of acidic silica sol and the bentonite were 0.2 % and 0.1% respectively, the clarifying operation can not only reach a demand of precipitating protein and other impurities but also make up to economical benefit. From these results, it was concluded that obtained parameters could be used for production of the garlic fructan.