人参皂苷Rg1对过氧化氢诱导SK-N-SH细胞凋亡的保护作用

The protective effect of Ginsenoside Rg1 on SK-N-SH cells apoptosis induced by hydrogen peroxide

目的研究人参皂苷Rg1对过氧化氢(H2O2)诱导SK-N-SH细胞凋亡的影响。方法建立H2O2致SK-N-SH细胞凋亡模型,MTT比色法检测细胞活力;应用流式细胞仪检测细胞凋亡率;DNA梯型观察凋亡细胞的DNA片段化;化学比色法测定细胞培养液和细胞内丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;RT-PCR检测细胞bcl-2、bax的表达水平。结果100μmol/LH2O2诱导SK-N-SH细胞12h,细胞活力下降(均P<0.01),细胞凋亡率为16.6%,DNA断裂呈片段状,细胞培养液及细胞内MDA含量增加,SOD活性下降,细胞中bax表达明显升高。而人参皂苷Rg1可明显减少细胞损伤,使细胞中bax表达明显减少、细胞培养液及细胞内MDA含量减少、SOD活性增加、细胞凋亡的各项指标均明显改善。结论人参皂苷Rg1对H2O2诱导的SK-N-SH细胞凋亡具有保护作用,其作用机制可能与提高SK-N-SH细胞的抗氧化能力以及减少bax表达有关。

Objective To investigate the protective effect of ginsenoside Rgl on SK-N-SH ceils apoptosis induced by hydrogen peroxide in culture. Methods H2O2 induced SK-N-SH cells apeptosis model was established. The cells activity, apoptotic rate and DNA gragmentation were measured by MTT, flow cytometry and DNA ladder analysis. The contents of malondialdelyde （MDA） and superoxide dismutase （SOD） in the cells and culture medium were measured by chemochromatometry respectively. The expressions of target mRNA of bcl-2 and bax were measured by RT-PCR. Results The cell activity decreased remarkably induced by 100 μmol/L H2O2 for 12 hours （P 〈0. 01 ）, presenting apoptotic rate as 16. 6% and resulting in DNA gragrnentation, the increased MDA level and bax expressions and the decreased SOD level in cells and culture medium. Ginsenoside Rgl obviously reduced cell apeptosis, decreased the expression of gene bax and MDA level and increased SOD level in cells and culture medium, improving the apoptotic index. Conclusions Ginsenoside Rgl has protective effect on SK-N- SH cells apoptosis induced by H2O2, which may be related to inhibiting lipid peroxidation and decreasing the expression of bax.