乳腺成体干细胞体内及体外特性的实验研究

Experimental study of characters of adult mammary stem cells in vitro and in vivo

目的观察来源于乳腺癌患者的乳腺成体干细胞的体外生长特性。并将其移植到裸鼠体内,研究其体内的发展及变化趋势。方法以免疫磁珠分离乳腺成体干细胞进行体外培养。30只裸鼠,随机分为2组,以胸壁皮下细胞注射法移植入裸鼠体内,并以移植入MCF-7乳腺癌细胞的裸鼠作对照。于术后行大体观察,术后6w行组织学检查。结果乳腺成体干细胞在体外培养可以分化成导管上皮细胞及肌上皮细胞,将其移植裸鼠体内后形成的组织与肿瘤组织有明显差别。在组织切片中可见形成类似乳腺腺管样结构,未形成癌巢组织,并由结缔组织分隔成若干小叶,可见到大量的脂肪组织。结论经免疫磁珠分离的乳腺成体干细胞具有继续增殖及分化的特性,可以诱导裸鼠体内形成类似乳腺样组织,为乳腺形成及发育的研究提供一种新的方法。

Objective To observe the growth character of the mammary adult stem cells （MASCs） in vitro and to explore the development in vivo by mammary stem cells transplantation into nude mice. Methods MASCs were seperated by magnetic activated cell sorting （MACS） to culture in vitro. Thirty nude mice were divided into two groups randomly. MASCs and MCF-7 cells were injected subcutaneously respectively in the axilla of mice as MASCs group and control group. The body observation was done after operation and histological examination was conducted 6 weeks after operation. Results MASCs cultured in vitro differentiated into luminal epithelial cells and myoepithelial. There was significant difference in tissues between MASCs group and MCF-7 group. The similar lacteal glandular tube structure seen in the histologeial section did not form cancer nest tissue, Was separated into several folials by connective tissue; and a great quantity of adipose tissue were be seen. Conclusions MASCs separated by MACS can induce nude mice to form mammary tissue analog, having characters of proliferation and differentiation, which provide a new method for the research of the formation and development of mammary gland.