浙江省1995年脊髓灰质炎实验室监测

LABORATORY SURVEILLANCE OF POLIOMYELITIS IN ZHEJIANG PROVINCE IN 1995

浙江省１９９５年共检测急性弛缓性麻痹（ＡＦＰ）病例２１２例，双份粪便标本１４天内采集率为７５．２％。脊髓灰质炎（脊灰）病毒分离阳性６例，分离率２．８％，其中Ⅰ型Ⅰ例，Ⅱ型３例，Ⅲ型２例，经型内鉴定均为疫苗株。自１９９１年以来已连续４年未分离到脊灰野病毒。２１２例ＡＦＰ病例粪便标本中分离出其他肠道病毒１９例，分离率９．０％。不同月份、不同年龄组、不同采便时间，其他肠道病毒的分离率虽不同，但无显著性差异。全省１９９５年共确诊临床符合病例３例，２例采集双份粪便标本，仅１例为合格标本，病毒分离结果均为阴性。

total of 212 AFP caes were detected in Zhejiang Province in 1995.The paired fecal specimen collecting rate within fourteen days was 75.2%.The positive isolation rate of polioviruses was 2.8%(6/212)Among them,Ⅰ was polioⅠ,3 were polioⅡ,and 2 were polio Ⅲ.Five of the six polio-viruses isolated were the vaccine strains as determined by intratypic differentiation using PCR-RFLP technique.NO wild-type poliovirus was isolated since 199 1.19 enteroviruses were iso-latat from 212 AFP caes,the isolation rate was 9.0%.No significant difference was found in theisolation late of enteroviruses according to ages,month of feces collecting and days of fecal speci-men delivery,though small difference existed.There were 3 cliniclly diagnosed polio coincidentcases in 1995 in the province,two paired fecal specimens were collected,one of them was unqualified,and no poliovirus was isolated.