HPLC测定葛根素微乳中葛根素的含量

Content Determination of Puerarin in Puerarin Microemulsion by HPLC

目的:建立HPLC测定葛根素微乳中葛根素的含量。方法:色谱柱为Shim-pack VP-ODS(4.6 mm×150 mm),流动相为甲醇-1 g/L枸橼酸水溶液(25∶75),测定波长250 nm,流速0.8ml/min,柱温为40℃。结果:葛根素与辅料及溶剂峰分离良好,在5.29-132.25μg/ml时线性关系良好(r=0.999 95)。结论:HPLC测定葛根表方法便捷、准确、重复性好,可用于葛根素微乳含量的测定。

Objective ： To establish an HPLC method for determining the content of puerarin in Puerarin Microemulsion. Methods： Shim-pack VP-ODS column （4.6 mm × 150 mm） was employed, and the mobile phase consisted of methanol-1.0 g/L acid citric （ 25： 75 ）. The detective wavelength was set at 250 nm. The flow rate was 0.8 ml/min, and the column temperature was at 40℃. Results： There was good resolution between puerarin and the exeipients under this chromatographic condition. The calibration curve was linear in the range of 5.29 - 132.25 μg/ml（ r = 0. 999 95）. Conclusion ： The method is simple and accurate in determining the content of puerarin in Puerarin Mieroemulsion.