携带IL-1B等位基因2牙周炎患者基础治疗后牙周可疑致病菌的变化

Change of Putative Periodontopathic Bacteria in Periodontitis with IL-1B Allele 2 after Basic Treatment

【目的】了解携带IL-1B(+3954)等位基因2的牙周炎患者在牙周基础治疗后龈下菌斑中牙周可疑致病菌的动态变化情况。【方法】选择了携带IL-1B(+3954)等位基因2牙周炎患者的42颗患牙为实验组(B2+group),无携带该等位基因牙周炎患者的40颗患牙为对照组(B2-group)。收集两组患牙在牙周基础治疗前、治疗后1、3、6个月时牙周临床指标菌斑指数(PI)、牙龈指数(GI)和探诊深度(PD),采集龈下菌斑,采用细菌培养鉴定方法测定牙周可疑致病菌的检出率和检出量(CFU/g)。【结果】(1)在同一观察时间点,两组的临床牙周各项指标无统计学差异。(2)基线时,实验组和对照组的牙周可疑致病菌检出率无统计学差异,而实验组的梭杆菌、类杆菌和BPAR检出量则较对照组高(P<0.05)。(3)基础治疗后1个月,两组的检出率和检出量明显下降(P<0.05)。(4)治疗后3个月时,两组的可疑致病菌检出率和检出量较1个月均有上升,且实验组的BPAR检出率和检出量均较对照组高(P<0.05)。(5)治疗后6个月时,两组的检出率基本回复基线水平;而实验组的真杆菌、梭杆菌、类杆菌和BPAR检出量则较对照组高(P<0.05)。【结论】(1)对于PD≥6mm的牙周炎患牙,基础治疗只能暂时控制牙周可疑致病菌。(2)基础治疗前后,携带IL-1B(+3954)等位基因2患者的牙周深袋处都可检出有更多的梭杆菌、类杆菌和BPAR。

[Objective] To study the dynamic changes of putative periodontopathic bacteria in subgingival plaques in periodontitis with IL-1B allele 2 after periodontal basic treatment. [Methods] The experimental group （B2＋group） were 42 teeth in periodontitis with IL-1B（＋3954） allele 2. The control group （B2-group） were 40 teeth in perioduntitis without the allele. The clinical indexes （plaque index, gingival index, and probing depths） in two groups were collected before and 1, 3, 6 months after periodontal basic treatment. The detective rate and detective amount （CFU/g） of putative periodontopathic bacteria in subgingival plaques were assayed by cultivation. [Results] （1） At the same observing time, the clinical indexes in two groups had no statistical difference. （2）The detective rates in two groups had no statistical difference on baseline. The detective amount of Fusobacterium, Bacterioides and BPAR in the B2＋ group were larger than those of B2- group （P〈 0.05）. （3） 1 month after basic treatment, thedetective rates and detective amounts of putative periedontopathic bacteria in two groups decreased obviously （P〈 0.05）. （4） The detective rates and detective amounts of putative periedontopathic bacteria in the two groups increased 3 months after basic treatment compared with that 1 month after, as well as that those of BPAR in B2＋ group were significantly higher than that of B2- group. （5） 6 month after treatment, the detective rates in the two groups almost returned to baseline; The detective amount of Eubacterium, Fusobacterium, Bacterioides and BPAR in the experimental group were larger than the control one （P〈 0.05）. [Conclusions] （1） Basic treatment could control the putative periedontopathic bacteria in the periodontal pocket with PD≥6mm for a while. （2） Fusobacterium, Bacterioide, and BPAR in putative periedontopathic bacteria were easily detected in the deep periodontal pocket in periedontitis with IL-1B （＋3954） allele 2.