低浓度玉米小斑病菌T毒素对玉米叶片苯丙氨酸解氨酶活性的诱导研究

Initiatory Discuss on Induced Resistance by the Filtration of Bipolaris maydis Race T Toxin Cultivation

分别用系列低浓度玉米小斑病菌T小种毒素处理玉米叶片后再接高浓度毒素观察其病斑大小,并测定了与抗病相关的关键酶——苯丙氨酸解氨酶(PAL)活性变化,从而确定了低浓度T毒素能够作为激发子来诱导玉米的系统获得性抗性具有一定的广泛性,且不同的品种所需的最适合浓度不同。对TB37的处理中0.025μg/mL组处理效果最好,PAL酶活性最高;而对TMo17的处理中0.05μg/mL组处理效果最好,PAL酶活性最高。

The low concentration of Bipolaris maydis race T toxin as an elicitor is extensive and can be repeatable We detained the same results on CMS-TB37 and CMS-TMo17 lines. At the same time, we measured the dimension of the leaf spot and determined the changes of PAL activities. Accordingly, the fight concentration of different breeds is discriminative .The effect of 0.025 μg/mL in different disposal of TB37 is better, but in TMo17 0.05μg/mL is.