地塞米松、利多卡因、对比剂对胶原酶活性影响的实验研究

The experimental study of the effect of dexamethasone,lidocaine and contrast medium on the activity of collagenase

目的研究激素类药物(地塞米松)、麻醉药物(利多卡因)、对比剂(欧乃派克)是否会影响胶原酶的溶解活性。方法取相同质量的椎间盘髓核组织,用相同浓度的胶原酶进行溶解。设对照组(未加药物)和实验组,不同实验组分别加入一定量的药物(地塞米松、利多卡因、欧乃派克)。24 h后通过检测各组溶液中羟脯氨酸含量测定胶原酶的溶解效果。统计分析对照组和实验组的差异。结果①地塞米松组、利多卡因组、欧乃派克组溶液中的羟脯氨酸含量明显低于对照组,尤其以利多卡因组为甚,P值分别＜0．05和＜0．01。②地塞米松+利多卡因组;地塞米松+欧乃派克组;利多卡因+欧乃派克组;地塞米松+利多卡因+欧乃派克组溶液中的羟脯氨酸含量明显低于对照组低,同时也分别比地塞米松组、利多卡因组、欧乃派克组低,差异有统计学意义。结论地塞米松、利多卡因、欧乃派克可不同程度地抑制胶原酶的溶解活性,任何两者或三者同时使用会进一步加剧对胶原酶活性的抑制。因此在使用胶原酶治疗腰椎间盘突出症时,不要把这些药物与胶原酶同时使用,以免影响胶原酶的治疗效果。

Objective To study the effects of hormone, anesthetie and contrast medium on the activities of collagenase lysis. Methods Nuclear tissues were divided into equal amount for different groups and same units of collagenase were used for the lysis. The only difference in the control group from experimental groups was that there were no dexamethasone, lidocaine or omnipaque but existing in experimental groups. Twenty four hours later, the concentrations of the hydroxyproline were determined in different groups and the data were analyzed by statistical software with computer. Results 1. The concentrations of hydroxyproline in the dexamethasone group, lidocaine group and omnipaque group were significantly lower than that of control group, especially that of lidocaine group, P value was 〈0.05 or 0.01.2. The concentrations of hydroxyproline in the dexamethasone ＋ lidocaine group, dexamethasone ＋ omnipaque group, lidocaine＋omnipaque group and dexamethasone ＋ lidocaine ＋ omnipaque group were significantly lower than that of control group; and simultaneously lower in dexamethasone group, lidocaine group, omnipaque group respectively; P value was also 〈0.05 or 0.01. Conclusion Dexamethasone, lidocaine and omnipaque can individually inhibit the activity of collagcnase at different degrees, so they shouldn＇t be used together with collagenase in treating the lumber disc herniation.