弱精症男子与正常人精浆和精子膜尿激酶酶活性的研究

Comparative studies on the urokinase activation in human seminal plasma and on spermatozoa between infertile patients with asthenospermia and healthy fertile men

应用琼酯糖－纤维蛋白－平板法和抗人尿激酶多克隆抗体的免疫阻断法，分别测定了２０名正常生育力男子和２２例精子活力低下（弱精症）的男性不育症患者精浆中及精子膜尿激酶型纤溶酶原激活因子（ｕＰＡ）的酶活性。结果：弱精症组精浆ｕＰＡ活性为２１３４±１５８１ＩＵ／Ｌ，正常生育组精浆ｕＰＡ活性为３３６５±１８５９．５ＩＵ／Ｌ，两组间差异有显著性意义（Ｐ＜０．０５）。弱精症组精子膜ｕＰＡ活性为５．１３±３．８２ｍＵ／１０６ｃｅｌｓ，正常生育组精子膜ｕＰＡ活性为１０．１７±６．１８ｍＵ／１０６ｃｅｌｓ，两组间差异有极显著性意义（Ｐ＜０．００５）。精子膜ｕＰＡ活性值与精子活率、活力亦呈线性相关。提示精子膜ｕＰＡ酶活性与精子活力及生育力可能有一定关系。

Objectives: To compare the urokinase activation on spermatozoa and in human seminal plasma between infertile patients with asthenospermia and healthy fertile men. Design: Urokinase activation on spermatozoa and in seminal plasma of asthenospermia group (22 cases) and normal group(20 cases) were determined by agarose fibrin plate method and immunological identification test with polyclonal antibodies against human urokinase. Setting: Clinic in the Center of Reproductive Medicine,Tongji Medical University. Methods: Seminal plasma and lysates of spermatozoa were added into a specific plate consisting mainly of agarose and fibrin,and incubated for certain time.Their relevant dissolved circles on the plate were measured for urokinase activation and calculated using a urokinase standard graph. Main outcome measure: Urokinase activation. Results: In semianl plasma and on spermatozoa significantly lower levels of urokinase activation on spermatozoa were found in patients with asthenospermia(2 134±1 581 IU/L,5.13±3.82 mU/10 6 cells)as compared to healthy fertile men(3 365±1 859.5 IU/L,10.17±6.18 mU/10 6 cells),both P < 0.05 . A positive correlation was observed between urokinase activation on spe rmatozoa and sperm viability( r =0.65, P ≤0.000 1) ,as well as with sperm motility( r=0.48,P <0.005). Conclusion: It is indicated that urokinase activation on spermatozoa may be related directly to sperm motiliy and fertility.