正常生育力和精子活力低下不育者精子运动计算机辅助分析

Computer assisted sperm analysis of sperm motility in normozoospermic and asthenospermic semen

用精子活力分析仪对７３名正常生育力男性射出精子进行了精子动力学指标分析；用上述指标分析了２００例精子活力低下不育男性射出精子动力学指标及各项运动参数间相互关系；用己酮可可碱作用精子活力低下不育男性射出精子，观察精子各项运动参数改变。结果：（１）正常生育力组，前向运动精子在总运动精子中比例与精子活动率间不呈相关性；随精子活动率提高，快速相和慢速相精子分布分别逐渐增加和减少，精子ＶＡＰ、ＶＣＬ、ＶＳＬ、ＢＣＦ、ＡＬＨ增加，ＬＩＮ和ＳＴＲ降低。（２）精子活力低下不育组，随精子活动率下降，主要表现在运动速度下降，与正常组比较，精子前向运动能力和运动速度均有明显下降（Ｐ＜０．０５），但ＬＩＮ和ＳＴＲ不管在组内还是与正常组相比均无明显差别（Ｐ＞０．０５），反映出这种前向运动能力的减低是由速度下降引起，速度分布变化以中速相明显。（３）己酮可可碱能明显提高精子活力低下不育者精子的活动率，前向运动率和精子运动快速相的分布；对精子ＬＩＮ，ＳＴＲ影响不明显。

Objectives: To analyze motility parameters from normozoospermic and asthenospermic semen,and effects of pentoxifylline(PF) on asthenospermic semen Methods: Computer assisted sperm analysis(CASA).The system is HTM IVOS. Results: (1)In normozoospermic semen,the ratio of progressive sperm in the total motile sperm (ten classes gradations according to WHO) was not correlated with the percentage of motile sperm ( r=-0.0806,P >0.05).Following the increase of percentage of sperm motility,the population of sperm in the fast velocity group increased and that in the low velocity group decreased,sperm VAP,VCL,VSL,BCF and ALH increased,and LIN,STR decreased.(2)In asthenospermic semen,it presented a decreasing of sperm velocity following the decreasing of percentage of motility sperm.Compared with normozoospermic semen,the progressive motility and velocity of sperm in asthenospermic semen declined sharply( P <0.05),but there was no differences of LIN and STR between the groups in asthenospermic semen and even between asthenospermic semen and normozoospermic semen.This showed that the decline of sperm progressive motility was due to decreasing of velocity( P>0.05 ).Change of population in sperm motility was obviously found in medium velocity group.(3)PF treatment on asthenospermic semen significantly increased sperm motility percentage ( P <0.001),sperm progressive motility ( P<0.001 ), and sperm population in fast velocity group (P <0.001).The LIN and STR were not effected obviously by PF. Conclusion: In normozoospermic semen,the ratio of progressive sperm in the total motile sperm was not correlated with the percentage of motile sperm,but was correlated with the parameter of sperm velocity.The population of sperm in medium velocity group was relatively balanced.In asthenospermic semen,decline of sperm progressive motility was due to decreasing of velocity; population in velocity was clearly changed in medium velocity group.PF had effects on increasing sperm motility percentage,sperm progressive motility,and sperm population in the fast velocity group,but the LIN and STR of sperm were not effected by PF.