蝎毒及其分离成分对^(60)Co γ射线照射后骨髓粒-单系祖细胞的影响

Influence of katsutoxin and its extract on bone marrow colony-forming unit-granulocyte and monocyte following ^(60)Co gamma ray radiation

实验于2004-09/2005-12在郑州大学基础医学院和郑州大学第一附属医院完成。将60只昆明小鼠随机分成5组:①空白对照、单独放射组各15只,腹腔注射无菌生理盐水0.2mL。②蝎毒抗癌多肽组10只、蝎毒抗癌多肽+放射组10只,按选择配制的浓度腹腔给与蝎毒抗癌多肽0.2mL。③蝎毒分离成分Ⅲ+放射组10只,腹腔注射0.2mL一定浓度的蝎毒分离成分Ⅲ。两次给药间隔时间为5.5h,连续给药7d。最后给药后24h对小鼠进行60Coγ射线1次照射,源皮距80cm,照射剂量7.5Gy,剂量率0.27Gy/min。照射后继续给药7d。7d后取骨髓,进行粒-单系干祖细胞培养。结果显示,蛇毒抗癌多肽+放射组、蝎毒分离成分Ⅲ+放射组的集落数明显高于单独放射组[(32±5),(27±3),(2±1)个/孔,P<0.001]。

The experiment was performed at Basic Medical College and First Affiliated Hospital, Zhengzhou University from September 2004 to Decethmber 2005. Totally 60 Kunming mica were divided into 5 groups randomly： ①blank control group （n =15） and simple radiation group （n =15）, The mica were given 0.2 mL sterile saline by intraperitoneal injection. ②antineoplastic polypeptide from Buthus Martensii Venom （APBMV） group （n =10） and APBMV plus radiation group （n =10） received 0.2 mL APBMV according to prepared concentration by intraperitoneal injection, ③ Katsutoxin extract Ⅲ plus radiation group （n =10） received 0.2 mL katstoxin extract Ⅲ by intraperitoneal injection every other 5.5 hours for 7 days. After 24 hours from the last injection, the mice were endured ^60Co g ray radiation （80 cm, 7.5 Gy irradiation dose, 0.27 Gy/min dose rate）. Then katsutoxin extract Ⅲ was given same as above for 7 days. Then bone marrow was extracted to be cultured to colony-forming unit-granulocyte and monocyte （CFU-GM）. The findings showed that colony amount of APBMV plus radiation group and katsutoxin extract Ⅲ plus radiation group was obviously more than that of simple radiation group [（32±5）, （27±3）, （2±1）pieces/well, P 〈 0.001].