摘要
目的:干细胞具有增殖能力和多向分化潜能,其成神经细胞特性为脑缺血神经细胞的保护及修复带来希望。文章回顾成体干细胞在中枢神经系统疾病修复中的研究和进展。资料来源:应用计算机检索Medline1997-01/2006-01期间的相关文章,检索词为"stem cells,cerebral ischemia",并限定文章语言种类为English。同时计算机检索中国期刊全文数据库1997-01/2006-01期间的相关文章,检索词为"干细胞,脑缺血",并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:①文章所述内容应涉及干细胞与脑缺血方面的研究。②选取有对照组的文章。③同一领域的研究选择近期发表或在权威杂志发表的文章。排除标准:重复研究的文章。资料提炼:共收集到187篇相关文献,86篇文献符合纳入标准,排除的101篇文献为内容陈旧或重复。符合纳入标准的86篇文献,涉及干细胞的分类以及干细胞在脑缺血损伤中的应用基础、途径、可能的作用机制等方面内容,选取其中的41篇作为文章参考文献。资料综合:干细胞是具有自我更新和多向分化潜能的细胞群体,可发育为某一器官或组织的特性为其在中枢神经系统疾病的保护与修复中的研究及应用奠定了基础。成体干细胞是多种组织中保留有未完全分化的原始细胞,具有横向分化特性,可分化为神经细胞,替代脑缺血损伤后丢失的神经元和神经胶质细胞,重建具有功能的神经环路;且成体干细胞可为梗死灶周围缺血半暗带组织提供营养支持,以及能促进内源性前体细胞的存活、迁移和分化,被认为是脑缺血最有希望的治疗。此外,成体干细胞可避免胚胎干细胞应用的伦理问题和免疫排斥障碍,是脑缺血损伤后神经保护和修复所需的理想干细胞供体。结论:干细胞的多向分化潜能为脑缺血损伤后的神经修复开辟了新的途径,其在脑缺血损伤后的神经细胞保护和修复以及功能重建的研究方面已取得相当进展,被认为具有广阔的应用前景,与之相关的问题均有待于进一步的研究。
OBJECTIVE: Stem cell has ability in proliferation and differentiation, and the property of its neuroblast brings the hope of the protection and recovery of neurocytes injury caused by cerebral ischemia. To review the research and advancement of adult stem cells in recovery of central nervous system diseases. DATA SOURCES: Using the key words of "stem cells, cerebral ischemia', we searched the Medline database of relevant articles publish'ed between January 1997 and January 2006 through computer in English. Meantime, using the same key words, we searched Chinese Journal Full-Text Database (CJFD) for relevant articles published in the same period in Chinese. STUDY SELECTION: All articles were selected firstly, and then citation of each record was reviewed. Inclusive criteria: ①The articles were involved in the study of stem cell and cerebral ischemia. ②The articles containing the control group were collected. ③As to the articles of the same field, those which were published in authoritative journal or published recently were collected. Exclusive criterion: The articles with repetitive research were excluded. DATA EXTRACTION: A total of 187 articles were selected, and about 86 among these articles met with inclusive criteria, other 101 articles that were much former or repeated were excluded. The 86 articles, which were met with inclusive criteria, were involved in the basis, way and possible mechanism of stem cells in cerebral ischemia, and 41 among these 86 articles were selected as the references. DATA SYNTHESIS: Stem cell was the cell colony, which could perform the abilities of self-renewal and multi-directional differentiation. The protection and recovery of neurocytes injury caused by cerebral ischemia in recovery of central nervous system diseases were based on stem cells characteristics, which could develop into certain organs and tissues. Adult stem cells were the archaeocytes remained in several tissues and performed the property of transversal cell differentiation. Neurocytes could be differentiated from adult stem cells and substitute nerve cells and neurogliocytes lost injury of cerebral ischemia, then to rebuild the nerve circuit loops that could perform certain function. Adult stem cells could also provide nutritional supports for tissue of semidarkness area around infarction and promote survival, immigration and differentiation of endogenous precursor cells, and the treatment were known as the most hopeful strategy. In addition, moral principles and immunologic rejection caused by the application of embryonic stem cells could be avoided. Therefore, adult stem cells were seemed as the ideal stem cell donor for nerves protection and recovery of neurocytes in cerebral ischemia injury. CONCLUSION: Multi-directional differentiation of stem cells develops a new way for nervousness recovery after cerebral ischemia injury, and considerable progression in nerves protection and recovery of neurocytes caused by cerebral ischemia injury and its functional reconstruction have got in the related studies. The application of stem cells in cerebral ischemia seems to be perspective, and other problems related to these fields deserve further studies.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第11期2144-2147,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(C03050311)
河南省高校创新人才培养基金(2000-10)~~