^(60)Co-γ射线对草莓组培苗诱变效应

Effects of ^(60)Co-γ rays on in vitro mutagenesis of strawberry

开展以20~100Gy60Co-γ射线辐照处理丰香、章姬两品种试管苗辐射诱变育种试验.试验结果表明各处理对草莓植株的生长和发育均有影响,但不同品种对60Co-γ辐射敏感性不同,半致死剂量也不同,丰香半致死剂量约为55Gy,章姬的半致死剂量约为64.4Gy;60Co-γ辐射对丰香品种有明显的抑制作用,主要表现在植株变矮,开花延迟,花朵数减少,且随着剂量增大,这种趋势更加明显;不同60Co-γ辐射处理对章姬开花影响较对生长影响更为明显,单从较高变异率和优变情况来看,20Gy处理变异率最高,达到36.53%,且优变多,是章姬品种60Co-γ辐射育种的较适宜剂量.

Two strawberry cultivars （Toyonoka, Akihime）in vitro were irradiated by ^60 Co gamma rays from 20 Gy to 100 Gy for induced mutation breeding. The results indicated that all treatments had effects on the growth and development of strawberry, but different cultivars had different sensitivity to the irradiation of gamma rays. And the LD50 was also different, which was 55 Gy for strawberry cultivar ＇Toyonoka＇ and 64.6 Gy for strawberry cultivar ＇Akihime＇. Inhibition was obviously produced by ^60Co gamma rays for strawberry cultivar ＇Toyonoka＇, which the plants became short in height, blooming time was put back, and the number of flowers was reduced, and this inhibition was more obvious with increase of the irradiation dose. The effect of ^60Co gamma rays on flowering was more distinct than that on growth for strawberry cultivar ＇Akihime＇. As tO the high aberrance and the excellent aberrance, the most suitable dose to strawberry cultivar ＇Akihime＇ was 20 Gy, at which the plants had high aberrance percent by 36.53% and much excellent aberrance.