低压稳恒直流电场对组织间液的电化学作用

Electrochemical Action of the Steady Direct Current Electrical Fields on the Interstitial Fluid

目的:探讨低压稳恒直流电场对组织间液的电化学作用。方法:中国家兔10只,经心脏直接采取已肝素化动脉血代替组织间液加入希托夫管中施以4.5 V(n=5)或6.0 V(n=5)稳恒直流电电解1 h。留取正常血液及电解完毕后阴极管、阳极管和中间管血液各2 mL,分别测定其pH值及HCO3-、K+、Na+、Cl-、Ca2+等离子浓度。结果:两种电压对pH值及上述电解质浓度影响无统计学差异;K+浓度中间管组较正常组高(P<0.01),但均在正常值范围;与中间管组比较,pH值在阴极管组升高(P<0.01),阳极管组降低但无统计学差异,也均在正常值范围;HCO3-、Na+、Cl-、Ca2+等在各组间均无统计差异。结论:6.0 V以下稳恒直流电对组织间液的电化学作用尚属安全范围。

Objective： The purpose of study was to investigate the electrochemical action of the applied weak steady DC EFs on the interstitial fluid. Methods： The heparinized arterial blood directly drawn from the left ventricles of hearts in 10 rabbits was added into the self-made Hittorf measuring device for determination of transference numbers instead of the Interstitial fluid of which electrolytes＇ character and concentrations are similar to the blood plasma, and then the direct current of 4.5 V（n=5） and 6.0 V （n=5）were applied to electrolyse for l h, respectively. The blood pH value and the ion concentrations of kalium （K^＋）, sodium（Na^＋）, chlorinum（Cl^-）, calcium （Ca^2＋）aod bicarbonate（HCO3-） were measured in the Hittorf device＇s anode cell, cathode cell and in-between cell, and the normal control. Results： The effect of the direct current of 4.5 V versus 6.0 V on the blood pH value and all ion concentrations showed no significant difference. K^＋ concentration in the in-between cell was higher than that in the normal control （P〈0.01）, but both were within the range of the normal value. Compared with the in-between cell, the pH value was higher in the cathode cell（P 〈 0.0 1）, and lower in the anode but showing no significant difference （P〉0.05）; it was also not out of the normal value leve. Moreover, compared with the Hittorf device＇s cells one another ,the concentrations of Na^＋, Cl^＋, Ca^2＋ and HCO3^- were not significant difference. Conclusions： The electrochemical action of the applied weak steady DC EFs （≤6.0 V） on the interstitial fluid was safe.