次声对HL-60白血病细胞株生长的影响

The effect of infrasound on the proliferation of HL-60 cells

目的:探讨次声治疗仪对HL-60人白血病细胞株生长的影响。方法:采用次声治疗仪发生的相同强度(档位3)不同作用时间的次声直接作用于离体培养的HL-60细胞(作用组),对照组细胞暴露在空气中,分别在实验处理的15min、30min、60min、90min和120min取样检测,以台盼蓝活细胞计数,MTT比色法及流式细胞仪技术,观察比较HL-60细胞的生长情况。结果:不同次声作用时间对HL-60细胞生长影响的差异不具显著性意义(P>0.05)。结论:治疗剂量的次声处理对HL-60细胞的生长无明显影响。

Objective： To study HL-60 cells＇ proliferation that were exposed to infrasound field generated by a therapeutic infrasound generator. Method：The vitro cultured HL-60 cells of treatment groups were exposed to infrasound-field generated by a therapeutic infrasound generator （with selection button 3）, the cells of control groups were exposed to air. The proliferation of the cells would be measured respectively at 15min., 30min., 60min., 90min and 120min. after the treatment. The cell viability would be showed by trypan blue staining, the cell multiplication measured by MTT assay, and the apoptosis of the cells detected with flow-cytometry. The results from each experimental condition were compared to those from appropriate control conditions. Result：The differences of the cell proliferations in all experimental conditions were not significant （P〉0.05）. Conclusion：HL-60 cells＇ proliferation were not significantly alerted by a therapeutic infrasound field.