不同强度脉冲电磁场对去势大鼠股骨骨钙含量的影响

Effect of pulsed electromagnetic fields of different magnetic intensity on bone calcium content of femur in ovariectomized rats

目的:通过不同强度低频脉冲电磁场干预去势大鼠骨质疏松模型,测定各组大鼠的股骨骨钙含量,探索治疗骨质疏松最适合的磁场强度。方法:按随机分组原则用密闭信封法将雌性3月龄SD大鼠50只分为5组:Sham对照组10只、OVX对照组10只、OVXⅠ组10只、OVXⅡ组10只、OVXⅢ组10只。除Sham对照组以外,对所有动物按文献方法切除双侧卵巢去势造模。OVXⅠ组、OVXⅡ组和OVXⅢ组三组大鼠每天在频率为8Hz,强度分别为0.77mT、3.82mT和9.87mT的磁场环境中治疗40min,共30d。Sham对照组和OVX对照组不进行干预。各组动物均在满30d后股动脉放血处死,取左侧股骨作骨钙含量测定。结果:OVX对照组大鼠的股骨骨钙含量显著低于其他4组大鼠(P<0.05或P<0.01)。结论:在磁场频率一致和干预时间相同的前提下,本文所用三种不同磁场强度的PEMFs治疗具有增加去势大鼠股骨骨钙含量的作用(P<0.01或P<0.05),3.82mT的PEMFs较0.77mT的PEMFs更能增加股骨骨钙含量。

Objective：To observe the effect of PEMFs of different intensity on bone calcium content of femur in ovariectomized rats,so as to find out the intensity for the best therapeutic efficacy;Method：Fifty female SD rats were randomly divided into five groups： （1）Sham-operated control; （2）Ovariectomized control; （3）Ovariectomized Ⅰ ; （4）Ovariectomized Ⅱ ; （5）OvariectomizedⅢ. Except 10 rats of the sham-operated control group,a standardized ovariectomy was administered in every rat. Bone calcium contents of femur were assessed after 30 days of PEMFs treatment. Result：The bone calcium contents of the ovariectomized control group were significantly lower than all the other groups（P〈0.05 or P〈0.01）, the differences of bone calcium contents between each of the other groups were not significant（P〉0.05）. Conclusion： Under certain field frequency（SHz） and time（40min/d×30d）, PEMFs of three different magnetic intensity reported can significantly elevate the bone calcium contents of femur to exceed or approach the normal level in ovariectomized rats. The effect of 3.82mT PEMFs is better than that of 0.77mT PEMFs.