电针对高血脂合并脑缺血大鼠治疗前后血脂含量变化的影响

Electro-acupuncture in use of treating hyperlipemia with cerebral ischemia disease

目的:研究电针对高血脂合并脑缺血大鼠治疗前后血脂含量的变化,为临床针灸治疗高血脂合并脑缺血疾病提供实验室数据及对策。方法:采用FeCl3化学诱导大脑中动脉血栓闭塞模型法,将高脂血症大鼠造成高血脂合并脑缺血模型,电针术前干预及脑缺血后全程治疗,通过酶法及选择性沉淀法测定治疗前后血脂。结果:治疗前CHO、TG、HDL-C及LDL-C血脂四项,各组分别与正常对照组比较,各组均有极显著性差异(P<0.01);血中CHO、TG、LDL-C升高和HDL-C降低,发生血脂异常现象。治疗后,与高血脂合并脑缺血模型组比较,高血脂合并脑缺血模型治疗Ⅰ组的CHO、TG降低有显著性差异(P<0.05),HDL-C升高没有显著差异(P>0.05)、LDL-C降低有极显著性差异(P<0.01);高血脂合并脑缺血模型治疗Ⅱ组的LDL-C降低有显著性差异(P<0.05);血中CHO、TG、LDL-C降低,HDL-C升高,高血脂合并脑缺血模型较早电针治疗Ⅰ组比高血脂合并脑缺血模型较迟电针治疗Ⅱ组更好。结论:电针可使高血脂合并脑缺血大鼠CHO、TG、LDL-C含量降低,HDL-C含量升高,有效地调节血脂水平。电针介入治疗时间点有其重要性,早期介入治疗能更有效的控制高血脂合并脑缺血损伤后的血脂水平,疗效更好,提示电针有效地调节血脂水平,可能是电针抗高血脂合并脑缺血损伤的重要机制之一。

Objective：To offer laboratory data and strategy for clinic to treat hyperlipemia with cerebral ischemia disease by electro-acupuncture（EA） therapy. Method： The model of hyperlipemia with cerebral ischemia was established by middle cerebral artery thrombosis （MCAT）. Enzymic method and selectivity precipitation method were used to detect the level of plasma lipid of the rats. Result： Before treatment, compared with the normal group, the level of CHO,TG and LDL-C increased （P〈0.01） and the level of HDL-C decreased （P〈0.01）. After treatment, compared with the hyperlipemia with cerebral ischemia group, in the hyperlipemia with cerebral ischemia Ⅰ-earlier treatment group, the level of CHO and TG all decreased （all P〈0.05）,the level of HDL-C increased （P〉0.05） and the level of LDL-C decreased （P〈0.01）; In the hyperlipemia with cerebral ischemia Ⅱ -later EA treatment group, the contents of LDL-C decreased （P〈0.05） and the contents of HDL-C increased （P〉0.05）, the level of CHO,TG and LDL-C increased and the level of HDL-C decreased. Conclusion： EA can decrease the level of CHO,TG,LDL-C and increase the level of HDL-C of hyperlipemia with cerebral ischemia rats significantly. Time of intervention is very important during using EA. EA can be used to control plasma lipid of hyperlipemia with cerebral ischemia significantly at the early stage. EA can up-regulate the plasma lipid, which may contribute to the effect of EA in improving hyporlipemia with cerebral ischemia.