己酮可可碱对人精子运动参数的影响

The effects of pentoxifylline on the movement parameters of human spermatozoa

为探讨室温下己酮可可碱（ｐｅｎｔｏｘｉｆｙｌｉｎｅ，ＰＦ）对人不同精子运动特性的影响，将正常精子（Ｉ组），少精及运动低下精子（ＩＩ组）和冷冻复苏精子（ＩＩ组）分别与ＰＦ培育３０分钟后洗涤，与对照组同时以计算机精液分析仪（ＣＡＳＡ）分析精子运动特性。结果：ＰＦ在室温下短时培育能提高各种精子的高活性运动（ＨＡ）。其作用在Ｉ、Ｉ组较明显；ＰＦ对平均轨迹速度（ＶＣＬ）、平均精子头摆动幅度（ＡＬＨ）及精子运动速度（ＶＡＰ）的升高作用和对平均直线性比值（ＬＩＮ）的降低作用经配对ｔ检验，具有显著和极显著意义（Ｐ＜０．０５及Ｐ＜０．００１），且持续至１５小时；室温下除ＬＩＮ以外的精子运动各参数与时间呈负相关，此变化大多于３小时后有统计学差异，以ＩＩ、ＩＩ组尤为明显。结果提示：室温下ＰＦ可提高精子高活性运动；在人工授精中精子准备时间以选择授精前３小时内进行为宜。

Objective: To observe the effect of pentoxifylline on the movement of human spermatozoa. Study Design: Normal spermatozoa(I), oligazospermia(II) and cryopreserved spermatozoa(III) were exposed to pentoxifylline (1mg/ml) for 30 min. at room temperature(20～25℃). The samples were examined with computer assisted sperm analyser at 0,1,3,6 and 15 hours after treatment with pentoxifylline. Results: The lateral head displacement(ALH), curvilinear velocity(VCL) and path velocity(VAP)were promoted and linearity(LIN) was decreased by exposure to pentoxifylline. When comparing with control groups, these changes were statistically significant (P<0.05 to P<0.001). The movement parameters, except LIN, of the spermatozoa were negatively correlated to the duration after ejaculation. The change was significant after 3 hours at room temperature. Conclusion: Pentoxifylline enhanced the hyperactivation of washed human spermatozoa. Three hours before insemination time should be choosen as the time for sperm preparation for ART.