摘要
目的:探讨血红素氧合酶-1(HO-1)抑制动脉粥样硬化进程的机制。方法:30只雄性SD大鼠随机分为正常对照组、模型组及氯血红素组。3月后观察主动脉形态学变化,ELISA法测定血中C-反应蛋白(CRP)及白介素-6(IL-6)水平,免疫组化法检测斑块内HO-1及巨噬细胞CD68的表达。结果:模型组及氯血红素组与对照组比较,CRPI、L-6、HO-1及CD68升高(P<0.05)。氯血红素组与模型组比较,主动脉内膜斑块面积占内膜面积比(RAAPI)显著减少(P<0.01),HO-1升高(P<0.01),CRPI、L-6及CD68降低(P<0.05)。结论:HO-1对动脉粥样硬化有明显的预防作用,这种作用可能是通过抑制炎症因子及巨噬细胞数量,减少炎症反应来实现的。
Objective： To explore the inhibition mechanism of heme oxygenase-1 （HO-1） on the progress of atherosclerosis in rats. Methods： Thirty male SD rats were randomly divided into three groups as control group, model group and hemin group. After three months, the serum concentrations of C-reaction protein（CRP） and interleulin-6（IL-6） of each group were measured. The expression of HO-1 and CD68 was measured by immunenhistochemistry. Results： Compared with that respectively in the control group, the CRP, IL-6, HO-1 and CD-68 in the model group and the hemin group increased significantly （P〈0.05）. Compared with that in the model group, HO-1 of the hemin group increased significantly （P〈0.01）, while the RAAPI, MDA, CD68 and MMP-9 decreased （P〈0. 05）. Conclusion： Heme oxygenase-1 can prevent atherosclerotic progression through inhibiting the inflammatory factors and decreasing the macrophages number, accordingly inhibiting the inflammatory response.
出处
《武汉大学学报（医学版）》
CAS
2007年第2期216-218,F0002,共4页
Medical Journal of Wuhan University
