前列腺素E_2对胆管上皮癌细胞增殖的影响

Effect of prostaglandin E_2 on the proliferation of human cholangicarcinoma cell

目的:探讨前列腺素E2(prostaglandin E2,PGE2)对胆管上皮癌细胞增殖的影响及其可能机制。方法:体外培养胆管上皮癌细胞株(HuCCT1),给予外源PGE2处理,以WST-1细胞增殖实验检测肿瘤细胞增殖率;Fluo-3/AM荧光负载胆管上皮癌细胞,激光扫描共聚焦显微镜检测细胞内钙离子浓度的变化情况;并用酶联免疫法测定细胞内环磷腺苷(cAMP)的浓度。结果:PGE2可促进胆管上皮癌细胞生长,而且可使细胞内钙离子和cAMP浓度升高。结论:PGE2影响胆管上皮癌细胞内Ca2+和cAMP浓度,可能与PGE2促进肿瘤细胞生长有关。

Objective：To investigate the effect of prostaglandin E2 on the proliferation of human cholangicareinoma cell and the possible mechanism. Methods：Human cholangicareinoma cell line （HUCCT1） was cultured and treated with PGE2, and the cell growth rate and viability was measured by cell proliferation assay with WST-1 regent. The intracellular free calcium concentration was measured using laser scanning confocal microscopy with fluo-3/AM as fluorescence probe. The level of cAMP was measured by immunoassay KIT. Results：PGE2 promoted the growth of cholangicarcinoma cell, and increased intracellular free calcium and cAMP in cholangicareinoma cell. Conclusion：The results that PGE2 could increase the concentration of intracellular free calcium and cAMP suggest that PGE2 may play a role in promoting the cell growth.