4’-甲醚-黄芩素对绒毛膜癌JAR细胞内Ca^(2+)浓度的影响

Effects of 4'-Methery-scutellarein on intracellular calcium concentration in human chorio-carcinoma JAR cell line

目的:探讨4′-甲醚-黄芩素对人绒毛膜癌JAR细胞的增殖抑制作用及细胞内Ca2+浓度的影响。方法:应用MTT法、流式细胞术和激光共聚焦扫描显微镜技术,体外观察该化合物对JAR细胞的影响。结果:4′-甲醚-黄芩素对JAR细胞的生长具有抑制作用。随着药物浓度与作用时间的增加,Annexin Ⅴ-FITC+/PI-细胞、细胞内Ca2+浓度逐渐增加,与对照组相比差异有显著性。结论:4′-甲醚-黄芩素能抑制人绒毛膜癌JAR细胞的增殖,诱导凋亡,其机制与提高细胞内Ca2+浓度有关。

Objective：To investigate the effects of 4＇-methery-scutellarein （4-MS） on proliferation and intracellular calcium concentration in human choriocareinoma JAR cell line. Methods：MTT assay, flow cytometry and laser scanning confocal microscopy were used to detect the effects of 4-MS on JAR cells. Results：4-MS inhibited the growth of JAR cells. Annexin V-FITC^＋/PI^- cells and intracellular calcium concentration were gradually increased with the increase of the dose and action time of 4-MS. There were significant differences compared with the control group. Conclusion ：4-MS can inhibit the proliferation and induce apoptosis of human choriocarcinoma JAR cell line. Its mechanism is related to the increase of intracellular calcium concentration.