重组降血压肽发酵培养基国产化的研究

Domestic Production Research of Fermentation Medium of Recombinant E.coli BL21 Expressing Antihypertensive Peptide

利用国产培养基原料在摇瓶发酵中对重组大肠杆菌E.coliBL21生产降血压肽的发酵培养基进行了研究,根据试验结果得出优化的国产培养基配方质量分数为:酵母粉3%,蛋白胨2.5%,葡萄糖1%,NaCl0.5%。选择使用优化国产培养基虽然比进口培养基的表达量下降了15%,但是生产成本大大降低,为下一步的工业化生产打下了基础。

The fermentation medium of the recombinant bacteria（ E. coli BI21 ） using domestic ingredients was studied. By Orthogonal test, the optimal culture medium was： yeast extract 3%, tryptone 2.5%, glucose 1% ,sodium chloride 0.5%. Although the expression of the domestic medium was 15% lower than the foreign one, the cost degraded greatly.