甲氧滴滴涕对雌性大鼠血清雌激素水平及卵巢抗氧化系统功能的影响

Effects of methoxychlor on serum level of steroid hormone and function of ovarian antioxidant system in female rats

目的:观察甲氧滴滴涕(MXC)对雌性大鼠血清雌激素水平、子宫指数(子宫质量mg/体质量g)及卵巢抗氧化系统的毒性.方法:雌性SD大鼠经口染毒MXC,随机分为50,100,200mg/(kg.d)3个染毒组和溶剂芝麻油组(对照组).阴道脱落细胞涂片法观察大鼠动情周期的变化;用放射免疫法测定各实验组大鼠血清雌二醇水平,测定大鼠体质量及子宫指数;分光光度法测定其卵巢超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力以及丙二醛(MDA)的含量.结果:各剂量MXC染毒组大鼠动情周期数明显低于对照组.50mg/(kg.d)组大鼠子宫指数较对照组增加.卵巢GSH-Px活力和SOD活力显著下降(P<0.05),而MDA含量明显升高(P<0.01).结论:MXC可致雌性大鼠动情周期紊乱,对卵巢的抗氧化系统产生一定影响.

AIM. To investigate the effects of methoxychlor （MXC） on serum steroid hormone, uterus index and ovarian antioxidant function in female Sprague Dawley rats. METHODS： MXC was administrated to the adult female rats at doses of 0 （control）, 50, 100 and 200 mg/（kg · d） for 21 d. Vaginal smears of rats were taken to determine estrous cycle. After treatment for 21 d, the level of serum estradiol was detected by radioimmunoassay in all the animals. Body weight and uterus index were also determined in all the animals. The activities of superoxide dismutase （ SOD ） and glutathione peroxidase （ GSH-Px） , and the level of malondialdehyde （MDA） were measured by spectrophotometry. RESULTS： The number of estrous cycle in MXC groups was obviously lower than that in control group （ P 〈 0.05）. The 50 mg/（kg · d）MXC significantly increased the uterus index. The activities of GSH-Px and SOD in the 3 MXC groups decreased significantly as compared with those in the control group （P 〈0.05）, but the level of MDA was raised in comparison with the control group （ P 〈 0.01 ）. CONCLUSION： MXC could disturb the estrous cycle and affect the function of ovarian antioxidant system in female SD rats.