维生素C和氨基胍对糖尿病大鼠肾功能的影响及机制研究

Mechanism research and effect of Vitamin C and Aminoguanidine on renal function of Streptozotocin-induced diabetic rats

目的观察维生素C、氨基胍对糖尿病大鼠肾功能的影响及机理。方法利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分为正常对照组、糖尿病组、维生素C治疗组、氨基胍治疗组、维生素C和氨基胍联合治疗组。治疗16周。观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌酐,内生肌酐清除率、24h尿蛋白排泄率、脂质过氧化物酶、超氧化物歧化酶。结果造模组大鼠均出现肾脏功能有损害;氨基胍、维生素C对血糖无影响,但能改善基本状况,降低糖尿病大鼠的尿素氮、血肌酐、24h尿白蛋白排泄率,脂质过氧化物酶,增加内生肌酐清除率、超氧化物歧化酶;两种药物联合用药具有协同作用。结论维生素C、氨基胍无降糖作用,但可通过抑制氧化糖基化对肾脏具有确切的保护作用,联合用药有协同作用。

[Objective] To observe mechanism research and the effect of VitaminC and Aminoguanidine on renal function Of Streptozotocin-induced diabetic rats. [Methods] Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were allocated to normal control group, model group, Vitamin C group, Aminoguanidine group, VitaminC and Aminoguanidine group. All rats were treated with corresponding drugs for 16 weeks. During and after the treatment, the general state, blood gloucose levels, blood urea nitrogen, serum creatinine, urinary albumin excretion rate of the 24-hours, malonaldehyde, erythrocuprein and clearance rate of creatinine in different groups. [Results] Diabetes mellitus and renal function lesion occurred in the four model groups. Aminoguanidine and Vitamin C have no effect on the blood gloucose levels, while they could improve the general state, decrease blood urea nitrogen, serum creatinine, urinary albumin excretion rate of the 24-hours, and malonaldehyde; increase clearance rate of creatinine and erythrocuprein. The two drugs have a cooperated effect.. [Conclusion] Aminoguanidine and Vitamin C have no effect in decreasing the blood sugar level but have a certain role in preventing renal function by inhibiting oxidation and glyeosylation. The two drugs have a cooperated effect.