烟粉虱B型和Q型群体遗传结构的RAPD分析

RAPD analysis of population genetic structure of Bemisia tabaci biotype B and biotype Q

近20年来,烟粉虱B型传入世界各地并暴发成灾,成为一种重要的农业入侵害虫;烟粉虱Q型则是近几年引起人们高度重视的一种新的入侵生物型,目前已传入许多国家并造成一定危害。本文利用RAPD分子标记对烟粉虱B型和Q型不同地理种群的遗传结构进行了分析。结果表明:(1)引物H16对烟粉虱B型不同种群扩增的特异带,能有效区分烟粉虱B型和Q型、浙江非B/Q型种群;(2)烟粉虱Q型种群各项遗传多样性指数均比烟粉虱B型的要高;(3)我国烟粉虱Q型来自伊比利亚半岛的可能性比来自中东地区的可能性要大。另外,聚类分析结果提示,RAPD分子标记能有效地区分烟粉虱不同生物型,但可能不适用于生物型之间亲缘关系分析。

Bemisia tabaci biotype B has been an invasive agricultural pest worldwide during the past two decades. Compared with the biotype B, biotype Q is a new invasive biotype. However, biotype Q has invaded into numerous countries and caused severe losses. The genetic structure of biotype B and biotype Q was analyzed using random amplified polymorphic DNA （RAPD） markers here. The results showed that ： （ 1 ） the specific amplified patterns using the primer H16 could distinguish biotype B populations from biotype Q populations and non-B/Q biotype population in Zhejiang province; （2）the genetic diversity of biotype Q was higher than that of biotype B ; （3） biotype Q more likely originated from Iberian peninsula than from Middle Eastern regions. Our results also suggest that RAPD markers seem to be unsuitable to be used in analysis of the relationships of different biotypes, though they could be used to distinguish the different biotypes.