摘要
利用凝集素细胞化学方法对短额负蝗Atractomorpha sinensis Bolivar配子发生过程中蓖麻凝集素(Ricinus communis agglutininⅠ,RCA-Ⅰ)受体分布进行了定位研究,旨在认识昆虫配子发生过程中β-半乳糖复合物的分布规律及意义。结果表明:在短额负蝗精子发生过程中,RCA-Ⅰ在各期生精细胞膜上均有明显的阳性反应,变形期及成熟期精子头部为中强度阳性反应。卵子发生中,各阶段卵母细胞均为阳性反应,在卵母细胞生长期阳性反应达峰值,随着卵黄颗粒的逐渐形成,阳性反应逐渐减弱,成熟卵子中卵黄膜上重新出现中强度阳性反应。结果提示短额负蝗配子发生过程中β-半乳糖复合物的高度表达和修饰变化与其配子发生密切相关。
For better understanding of the significance of β-gal glycoconjugates distribution during gametogenesis, the distribution of Ricinus communis agglutinin Ⅰ (RCA-Ⅰ) receptors during gametogenesis in Atractomorpha sinensis Bolivar was detected by using lectin-binding cytocbemistry method. The results showed that during spermatogenesis RCA-I receptor was found on the membrane of spermatogenic cells at different stages, and the heads of deforming spermatids and sperms showed moderate positive reaction. During oogenesis, there was RCA-Ⅰ positive reaction in the oocytes of all stages, and the quantity of positive granules reached the maximum in the oocyte growth phase. With the formation of yolk granules, the products of RCA-Ⅰ positive reaction diminished gradually in oocytes, yet on the yolk membrane of maturing egg there appeared moderate positive reaction again. The results suggest that β-gal glycoconjugates may play great roles during gametogenesis of A. sinensis.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第3期297-303,共7页
Acta Entomologica Sinica
基金
陕西省自然科学基金项目(2005C125)
关键词
短额负蝗
精子发生
卵子发生
糖复合物
蓖麻凝集素
Atractomorpha sinensis
spermatogenesis
oogenesis
glycoconjugates
Ricinus communis agglutinin Ⅰ