轻度子宫内膜异位症不孕患者腹腔液和血清一氧化氮水平变化及意义

Signification of nitric oxide concentration in peritoneal fluid and serum of women with early endometriosis-associated infertility

目的:探讨一氧化氮(NO)与轻度子宫内膜异位症(内异症)不孕发病的关系。方法:采用荧光法检测37例轻度内异症不孕患者(内异组)、28例输卵管性不孕组(管性组)和20例对照组血清和腹腔液的一氧化氮水平。结果:对照组、管性组和内异组腹腔液中一氧化氮代谢物水平F/F0分别为(1.1298±0.039251)、(1.136317±0.05875)和(1.205783±0.043172),内异组腹腔液中一氧化氮代谢物水平增高,与对照组和管性组比较,差异有统计学意义(P<0.05),管性组与对照组比较,差异无统计学意义(P>0.05);对照组、管性组和内异组血清中一氧化氮代谢物水平F/F0分别为(1.143167±0.045557)、(1.1334±0.026565)和(1.12725±0.0497),3组血清中一氧化氮代谢物水平比较,差异均无统计学意义(P>0.05)。结论:腹腔液NO水平变化可能与轻度子宫内膜异位症及轻度子宫内膜异位症不孕的发病有关。

Objective： To investigate the relation of nitric oxide （NO） and the pathogenesis of early endometriosis - associated infertility. Methods： The concentration of NO in peritoneal fluid and serum of 37 patients with early endometriosis - associated infertility （EMS group）, 28 patients with tubal infertility （Tubal group） and 20 patients without infertility （control group） were measured with 4, 5 - diaminofluorescein （ DAF - 2 ）, a fluorescence indicator of NO that emits fluorescence in response to a reaction with NO. Results： The F/ F0 which reflects the concentration of NO in peritoneal fluid of the control group , the tubal group and the EMS group respectively were （ 1.1 298 ±0. 039 251 ）, （ 1. 136 317 ±0. 058 752） and （ 1. 205 783 ±0. 043 172） . The F/F0 was statistically higher in the EMS group compared with the control group （ P 〈 0. 05 ） and the tubal group （ P 〈 0. 05 ） . But the F/F0 didnt seem to he different hetween the tubal group and the control group （P〉0.05）. The F/F0 in serum was （1. 143 167±0.045557） in the control group , （1.1 334±0.026565） inthe tuba/group and （ 1.12 725 ±0.0 497） in the EMS group. The difference of NO concentration without statistical significance among the three group （ P 〉 0. 05 ） . Conclusion： NO may play an important role in the pathogenesis of early endometriosis - associated and pathophysiology of endometriosis - associated infertility.