免疫斑点法检测脑脊液中白血病细胞相关脱落抗原CD_9方法建立及应用

DETECTION OF LEUKEMIA-ASSOCIATED ANTIGEN CD_9 IN CEREBRALSPINAL FLUID BY DOT-ELISA

在双夹心ＣＥＬＩＳＡ检测脑脊液白血病细胞相关脱落抗原ＣＤ９基础上，建立了更为简便快速的免疫斑点法（Ｄｏｔ－ＥＬＩＳＡ）。检测１６例初诊ＣＤ９阳性白血病患儿脑脊液，５例阳性，并且随着鞘注化疗，ＣＤ９滴度逐渐下降。初步研究结束表明：脑脊液ＣＤ９含量检测有助于鞘注化疗疗效监测。

Abstract:In order to develop a easier and more convenient method to detect leukemia-associatedantigen CD9 in cerebralspinal fluid, Dot-ELISA was successfully used on the basis of doublesandwich ELISA. The result showed that CD9 was positive in 5 of the 16 patients at diagnosis, and the titer of CD9 gradually decreased after intrathecal therapy was performed,.Thus. it indicated that the detection of CD9 in CSF was useful to evaluate the effects of intrathecal therapy