光合细菌球形红细菌厌氧降解氯代苯

Anaerobic degradation of chlorobenzene by phototroph Rhodobacter sphaeroides

采用光合细菌球形红细菌(Rhodobacter sphaeroides)在厌氧光照条件下对氯代苯进行生物降解.结果表明,氯代苯不能作为球形红细菌生长的唯一碳源和能源.球形红细菌厌氧降解氯代苯是在适宜碳源存在下,由氯代苯诱导产生诱导酶以共代谢的方式进行,降解途径是先打开苯环生成小分子的氯代烷烃、再还原脱氯.在培养基中加入一定量的酵母膏,可使细菌生长的停滞期明显缩短,提高氯代苯的脱氯率.在氯代苯浓度为100mg/L时,厌氧降解的最适宜条件为苹果酸浓度1.0g/L、硫酸铵浓度0.1g/L、pH7.0、酵母浸膏浓度1.0g/L.

Chlorobenzene was biodegradated under anaerobic illumination condition, adopting photosynthetic bacteria Rhodobacter sphaeroides, chlorobenzene would not be taken as sole carbon and energy sources. Rhodobacter sphaeroides anaerobic degradation of chlorobenzene was carded out with the cometabolism way of chlorobenzene induced enzyme in the presence of the suitable carbon source. It＇s degrading ways were first open chlorobenzene ring to form small molecular alkyl halide, then reductive dechlorination. Adding definite amount of yeast ointment in the culture medium could shorten obviously the lug phase of bacteria growth and enhance the dechlorination rate of chlorobenzene. When the concentration of chlorobenzene was 100mg/L, the most suitable conditions of anaerobic degradation were manlic acid concentration of 1.0g/L, ammonia sulfate concentration of 0.1g/L, pH value of 7.0 and yeast extract concentration of 1.0g/L.